Human Progranulin Quantikine ELISA Kit

R&D Systems | Catalog # DPGRN0

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (25 µL), Human Milk (10 µL)

Sensitivity

0.54 ng/mL

Assay Range

1.56-100 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
View all Progranulin/PGRN ELISA Kits »

Product Summary for Human Progranulin Quantikine ELISA Kit

The Quantikine Human Progranulin immunoassay is a 4.5 hour solid phase ELISA designed to measure Progranulin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Progranulin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Progranulin. Trypsin and Elastase cleaved Progranulin show reduced recognition in this assay, demonstrating specificity for Progranulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 13.5 32.7 64.8 14.4 33.1 64.4
Standard Deviation 0.7 1.9 4.1 1.0 2.3 4.4
CV% 5.2 5.8 6.3 6.9 6.9 6.8

EDTA Plasma, Heparin Plasma, Human Milk, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 15.5 36.6 70.8 15.7 35.8 69.1
Standard Deviation 0.9 1.7 3.1 1.3 2.9 5.1
CV% 5.8 4.6 4.4 8.3 8.1 7.4

Recovery for Human Progranulin Quantikine ELISA Kit

The recovery of Progranulin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 103-112
EDTA Plasma (n=4) 102 95-110
Heparin Plasma (n=4) 100 92-108
Serum (n=4) 104 90-115
Urine (n=4) 109 99-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Progranulin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Progranulin/PGRN ELISA Linearity

Scientific Data Images for Human Progranulin Quantikine ELISA Kit

Human Progranulin/PGRN ELISA Serum/Plasma/Urine Human Milk Standard Curve

Human Progranulin/PGRN ELISA Serum/Plasma/Urine Human Milk Standard Curve

Human Progranulin/PGRN ELISA Cell Culture Supernate/Saliva Standard Curve

Human Progranulin/PGRN ELISA Cell Culture Supernate/Saliva Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Progranulin/PGRN

Progranulin, also known as Acrogranin, PCDGF, and Epithelin/Granulin Precursor, is a widely expressed glycoprotein that can undergo proteolysis to generate numerous Granulin peptides. Progranulin plays a role in the regulation of mesothelial differentiation, macrophage development, sexual differentiation of the brain, cartilage proliferation, wound repair, and the development of rheumatoid arthritis and osteoarthritis. It is often upregulated in cancer and contributes to tumor progression. Progranulin upregulation or mutation is associated with several neurological disorders including frontotemporal dementia, Creutzfeldt-Jakob disease, Alzheimer's disease, and motor neuron disease.

Alternate Names

Acrogranin, GEP, GP88, GRN, PCDGF, PEPI, PGRN, Proepithelin

Entrez Gene IDs

2896 (Human); 14824 (Mouse)

Gene Symbol

GRN

Additional Progranulin/PGRN Products

Product Documents for Human Progranulin Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Progranulin Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

Citations for Human Progranulin Quantikine ELISA Kit

Customer Reviews for Human Progranulin Quantikine ELISA Kit (2)

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  • Human Progranulin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Supernatants of various in vitro cultured cancer cells
    Verified Customer | Posted 09/24/2023
    Supernatants of various in vitro cultured cancer cells.
    Human Progranulin Quantikine ELISA Kit DPGRN0
  • Human Progranulin Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 08/29/2017
    We used device loading with cells that can release progranulin. After certain time in in vivo we explant device and collect culture supernatant for the measurement of progranulin. The kit standard curve was very good as R2 was 1. We can easily detect in our samples.
    Human Progranulin Quantikine ELISA Kit DPGRN0

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Protocols

View specific protocols for Human Progranulin Quantikine ELISA Kit (DPGRN0):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well within 15 minutes. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human Progranulin Quantikine ELISA Kit

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  • Q: What isoforms does this kit detect?

    A: This kit detects isoform 1. R&D Systems has not been able to evaluate isoforms 2 and 3 in this kit.

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